Plasmid_Backbone

Part:BBa_K1480014:Design

Designed by: Jesus Gilberto Rodriguez Ceja   Group: iGEM14_UANL_Mty-Mexico   (2014-10-17)


pSB3C5 + After TALEN (K1480001) Target site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Illegal EcoRI site found at 2765
    Illegal XbaI site found at 2780
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2765
    Illegal NheI site found at 1403
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2771
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2765
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2765
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2765
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2780
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 994
    Illegal AgeI site found at 1317
    Illegal AgeI site found at 3566
    Illegal AgeI site found at 3678
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 289


Design Notes

Modifications where inserted by PCR on both directions due to TALEN specificity.


Source

Derived from pSB3C5 plasmid

References